Composite

Part:BBa_K592013:Design

Designed by: Lei Sun   Group: iGEM11_Uppsala-Sweden   (2011-09-18)


HO1-pcyA, no promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1564
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1117
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

B1001 instead of B0015 since this part was chosen to put into the bacterial chromosome using lambda-red recombination. The standard double terminator B0015 has extensive homologous sites within E.coli genome, therefore it's not used.

This part lacks promoter. What promoter to be used is up to the user. However, we recommend weak promoters due to HO1's enzymatic activity kills cells by consuming heme.


Source

Assembled using parts from the MIT 2011 iGEM distribution.

References